Why touchdown pcr

For more information on protocol and optimization, see references 1 and 2. I also found the Roux KH paper very useful for optimization in general. Has this helped you? Then please share with your network. This was an excellent post. It was clear, had references, and pointed out a pretty clear protocol. Thank you for the help! I am going to buy a new one. Is it good? Or too old? Thank you. Information on basic PCR can be found in any introductory molecular biology textbook or book on molecular biology techniques.

Indeed Kurt — hence my Australia comment. Since I am not a fan of acronyms, I think we can also note that this technique was published without one. You must be logged in to post a comment. This site uses Akismet to reduce spam. Learn how your comment data is processed. Facebook Twitter LinkedIn More. Written by Shoba. Log in to Reply. Jessica on February 22, at am. SantaLucia Jr. A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics.

USA 95 , — Improved nearest-neighbor parameters for predicting DNA duplex stability. Biochemistry 35 , — Sugimoto, N. Improved thermodynamic parameters and helix initiation factor to predict stability of DNA duplexes. Hecker, K. High and low annealing temperatures increase both specificity and yield in touchdown and stepdown PCR. Biotechniques 20 , — Zhang, Z. Single-tube heminested PCR coupled with 'touchdown' PCR for the analysis of the walleye dermal sarcoma virus env gene.

Methods 60 , 29—37 Rubie, C. Biotechniques 27 , —, Duckworth, A. The use of 'touchdown' polymerase chain reaction increases the sensitivity and specificity of t 11;14 q13;q32 detection in patients with mantle cell lymphoma. De la Horra, C. Fietto, J. Biotechniques 32 , —, — Piraee, M. Levano-Garcia, J.

Mapping transposon insertion sites by touchdown PCR and hybrid degenerate primers. Biotechniques 38 , — Kornmann, B. Ding, W.

Combining restriction digestion and touchdown PCR permits detection of trace isoforms of histamine H3 receptor. Biotechniques 39 , — Li, J. Ault, G. Methods 46 , — Roux, K. Strauss, W. Preparation of genomic DNA from mammalian tissue. Chomczynski, P.

The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on. Rozen, S. Primer3 on the WWW for general users and for biologist programmers.

Yuryev, A. PCR Primer Design ed. Humana Press, Totowa, NJ, Book Google Scholar. Brody, J. Biotechniques 37 , , , History and principles of conductive media for standard DNA electrophoresis.

Mokaddas, E. Molecular fingerprinting of isoniazid-resistant Mycobacterium tuberculosis isolates from chest diseases hospital in Kuwait. Ahmad, S.

Probes 18 , — The occurrence of rare rpoB mutations in rifampicin-resistant clinical Mycobacterium tuberculosis isolates from Kuwait. Agents 26 , — Species spectrum of nontuberculous mycobacteria isolated from clinical specimens in Kuwait. Harboe, M. Cross-reaction between mammalian cell entry Mce proteins of Mycobacterium tuberculosis. Mammalian cell-entry proteins encoded by the mce3 operon of Mycobacterium tuberculosis are expressed during natural infection in humans.

Download references. You can also search for this author in PubMed Google Scholar. Correspondence to John S Mattick. Reprints and Permissions. Korbie, D.

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